墨兰原球茎生长的研究

EFFECTS OF MEDIA, GROWTH REGULATORS ANDDIVIDING ON THE GROWTH OF CYMBIDIUM SINENSEPROTOCORMS CULTURED IN VITRO

摘要:

以墨兰种子胚经培养诱导萌发形成的原球茎为材料，研究其生长的最适条件。结果表明：最佳基本培养基是ＭＳ培养基；NAA 0.5 mg L^-1＋BA 1.0 mg L^-1 有利于生长和分化；在培养基中加入１％蔗糖和0.1％活性炭最有利于原球茎生长；pH5.0偏酸环境适合于生长；每天给予15.5 μmol m^-2s^-1 16 h的光照最佳。如将上述单因子组合在一起，其原球茎鲜重增长率比对照（Ms培养基）高46％。在不同切割方式中，掰开法鲜重增长率比纵切法或横切法都高。

Abstract:

The optimal culture conditions of the growth of Cymbidium sinense protocormsinduced from embryo were studied. The results showed that MS was the best basal mediumfor C. sinense protocorm growth and organogenesis. Treatment of 0.5 mg L^-1 NAA incombination with 1.0 mg L^-1 BA was most satisfactory for protocorm growth. When 1%sucrose and 0.1% activated carbon were added to the basal medium, a superior growth ofprotocorms was observed. Highest increase of the fresh weight of protocorms was at PH 5.0, Protocorms grew well under l5.5μmol m^-2s^-1 light intensity for 16 hours per day．Combined treatment with all optimal single factors mentioned above showed that the fresh weight of protocorms was 46％ higher than that of the control．As for the in crease of proliferation coeficient，dividing the protocorms by hands gave good result in cutting Inanner．