野生稻基因组随机扩增多态性DNA(RAPD)分析

RAPD ANALYSIS OF WILD RICE GENOMES

摘要:

用18个随机引物对2份栽培稻、12份包含有六个基因组型的野生稻DNA进行了扩增，共获得147个多态性DNA片断，把这些多态性DNA片断作为遗传位点用UPGMA法计算出各材料间的遗传相似性系数，并作了聚类分析．主要结果如下：1普通野生稻同栽培稻的亲缘关系很近，其中江永普通野生稻更接近于粳稻．2．CCDD组的Oryzalatifolia和EE组的O．australiensis遗传多态性相似。3．B、C、D、E组的遗传多态性相似，组成一个复合体，此复合体与A组的遗传多态性也相似，而F组则相距较远．4．O．mcyeriana和Rhynchofyzasabulata尚未确定组型，RAPD测定结果表明，前者与其它组型的种亲缘关系较远，后者则与AC复合体的种较近．

Abstract:

Fourteen DNA samples, of which 2 cultivated and 12 wild rices, were amplified for random amplified polymorphic DNA (RAPD) analysis with 18 random primers, and 147 polymorphic bands were obtained. The genetic similarity coefficient was calculated by UPGMA cluster analysis. Results showed that (1) Oryza rufipogon had close relation to cultivated rice, and Jiangyong wild rice was more closely related to Japonica, (2) The polymorphism of O. latifolia with CCDD genome was singular to that of O. australiensis wi...