芥蓝下胚轴离体培养及高频率植株的再生

PLANT REGENERATION IN FITRO FROM HYPOCOTYL EXPLANTS OF BRASSICA ALBOGLABRA

摘要:

三个芥蓝品种下胚轴离体植株再生的条件的研究结果表明：下胚轴切口处可直接诱导出芽，诱导“早花尖叶芥蓝”、“中花尖叶芥蓝”和“迟花尖叶芥蓝”直接出芽的最佳激素组合分别为2．0mgL^-1BA，0．3mgL^-1NAA＋2．0mgL^-1BA，0．5mgL^-1NAA＋2．0mgL^-1BA，其相应的芽发生频率分别为84．6％，86．7％，93．3％。诱导芽发生的最适蔗糖浓度是1％。培养基中加入4．0mgL^-1AgNO₃和500mgL^-1MES可显著提高芽再生频率。再生芽在MS附加0．1mgL^-1NAA的培养基上诱导生根形成完整植株。离体再生苗与种子萌发实生苗田间生长外形差别不大，但长势稍慢。

Abstract:

Plant regeneration from in vitro culture of hypocotyl in three cultivars of Brassica alboglabra was studied. The results showed that shoots could be induced directly from both ends of hypocotyl fragments with optimum auxins of 2.0 mg L^-1 BA for cultivar 'Early Flowering', 0.3 mg L^-1 NAA+ 2.0 mg L^-1 BA for' Middle Flowering' and 0.5 mg L^-1 NAA+2.0 mg L^-1 BA for' Late Flowering', the corresponding shoot-induction frequencies being 84.6%, 86.7% and 93.3%, respectively. The optimum concentration of sucrose for shoot-induction was 1%. Addition of 4.0 mg La AgNO₃ and 500 mg L^-1 MES in the medium could greatly increase the frequency of shoot regeneration. The plant let could well develope after rooting in half strength MS medium supplemented with 0.1 mg L^-1 NAA.No morphological differences were observed between in vitro plants and seedling plants.