促花和抑花处理对柑桔成花及芽肉蛋白质组份的影响

EFFECTS OF FLORAL PROMOTION OR INHIBITION TREATMENTS ON FLOWERING OF CITRUS TREES AND PROTEIN FRACTIONS IN BUDS

摘要:

对金柑、柑和温州蜜柑施行促、抑花调控处理，并利用SDS－PAGE和IEF－PAGE电泳技术，分析了金柑、柑和温州蜜柑在受促、抑花处理后芽体的蛋白质图谱变化动态。结果表明，在柑桔花芽诱导期，经GA₃或BA抑花处理的芽组织中均有新的小分子蛋白质或中性至弱碱性蛋白质出现。随着花芽由生理分化向形态分化过渡，环割处理芽中出现小分子或酸性特异蛋白质。可能表明柑桔成花或抑花具有不同的基因表达与蛋白质代谢特征。进一步证实了因处理时间不同，BA对柑桔花芽分化具有促进或抑制的双重作用．

Abstract:

Different methods of flowering regulation were used on oval Kumquat (Fortunella margarita Swingle), Ponkan (Citrus reticulata Blanco' Pokon' ) and Satsuma orange (C.reticulata Blanco' Miyagawa Wase') trees which were all grafted on rootstocks of trifoliate orange (Poncirous trifoliata) The changes in protein fractions during the flower initiation were determined by SDS-PAGE and IEF-PAGE. The results showed that flower numbers were obviously increased by girdling treatment and decreased by GA₃, and were also decreased by BA treated at the early stage of flower induction but increased by that at the late stage of flower formation. At the flower induction stage, some of the special proteins were observed in the buds treated with GA₃, but disappeared finally. In the flower primordium formation stage, the intensity of protein bands in the flower buds increased in the shoots girdled, and some special proteins were found in the flower buds. It is suggested that different methods of flowering regulation might result in different gene expression and characteristics of protein metabolism. Some protein with smaller molecules and lower isoelectric point were related to flower initiation of citrus trees.