Abstract:According to the published sequences, PCR primers of lundnocyclopropane-1-lcarboxylate (ACS) and l-aminocyclopropane-lcarboxylate oxidase (ACO) gene of tomato amplification were designed. Fragments of ACS and ACO gene were generated. The products of PCR fragments and T-vector ligation were transformed into E. colt (DH5a).The recombinants were selected by blue-white colony screening and restriction analysis.After partial sequencing and Southern hybridization, the fragments and stringed products o f two genes were subcloned into plant gene expression vector pBllZI in antisense orientation. The antisense vectors were transformed into Agrobaterium tumefaciens LBA4404 by freeze-thaw method. Fourteendayxild cotyledon pieces were infected with Agrobacterium,and transformants were selected with 75 ig nil-l kanamycin. Southern blotting with 0.gkb fragments of ACS gene as probe provided that two positively transgenic plantletS were obtained.
