火炬松胚性细胞悬浮培养物的生长参数变化

CHANGES IN GROWTH PARAMETERS OF EMBRYOGENIC CELL SUSPENSION CULTURES OF LOBLOLLY PINE (PINUS TEEDA)

摘要:

以火炬松（PinustaedaL.）成熟合子胚来源的胚性愈伤组织为材料建立了胚性细胞悬浮系，测定了其培养物的鲜重、干重、细胞体积和胚数及培养液中的pH值、电导率和蔗糖浓度等生长参数在培养过程中的变化动态。结果表明，在培养周期内，培养液中的pH值、电导率和蔗糖浓度的逐步降低与培养物的鲜重、干重、细胞体积和胚胎数的逐步增加保持一致性。在培养至18—21d，pH值、电导率和蔗糖浓度均接近或降到最低点，而胚数及细胞体积的增长都达到最高点。

Abstract:

Embryogenic calli derived from mature zygotic embryos of loblolly pine (Pinus taeda L.) of three genotypes from Hunan Province, viz. Shaoyanghigh-2, Yongshunmiddle-1 and Yongshunmiddle-3 were used in embryogenic cell suspension culture. Parameters in the growth of cultures, including fresh weight, dry weight, packed cell volume, somatic embryo number, as well as pH, conditivity, and sucrose concentration of the culture medium were measured during culture period. The results showed that, during culture circl...