海藻中清除氧自由基的物质

THE SUBSTANCES SCAVENGING OXYGEN FREE RADICALS IN MARINE ALGAE

摘要:

新鲜海藻的提取液含有超氧物歧化酶（SOD）活性物质，能清除超氧自由基（O2-）。海藻的SOD活性通常为60—280Ug-1FW，而在孔石莼（Ulvapertusa）、江蓠（Gracilariaverrucosa）和凤尾菜（G．eucheumoices）中活性较高，约为300Ug-1FW。一般来说，海藻的SOD活性和稳定性为：绿藻＞红藻＞褐藻。绿藻的SOD以CuZn-型为主，而蓝藻的SOD以Fe-型为主。以江蓠琼枝（Eucheumagelatinae）提取液作PAGE并SOD活性染色时，除了观察到SOD同工酶带之外，还发现在前沿指示剂附近有一区域，此区域与高效自由基清除剂SPD（Superphycodismutas）的电泳行为和对氮蓝四唑（NBT）负染色的抑制相同，可能两者为同一种物质。

Abstract:

Some substances with activity of superoxide dismutase (SOD) extracted from fresh algae can scavenge superoxide radical (O). The activity of SOD in algae is usually 60 - 280 U g-1FW, but that in Ulva pertusa, Gracilaria verrucosa and G. eucheumoides is higher (about 300 U g-1FW). In general, the activity and stability of SOD in algae are in order: green algae>red algae>brown algae. The main types of SOD in green algae are CuZn-SOD, but in blue algae, Fe-SOD. The result of PAGE and SOD active staining of the ...