茉莉酸甲酯对烟草幼苗抗炭疽病的影响

EFFECT OF METHYL JASMONATE ON RESISTANCE OF TOBACCO(NICOTIANA TABACUM CV. SAMSUM NN) SEEDLINGS TO COLLETOTRICHUM DESTRUCTIVUM O'GARA

摘要:

本文探讨了茉莉酸甲酯（MJ）对烟草幼苗抗炭疽病（由毁灭性刺盘孢引起）的影响。不同品种烟草抵抗毁灭性刺盘孢孢子侵染的能力不同，“巴西”品种的抗性强，“K326”品种抗性弱，“广黄55”品种抗性居中。MJ处理烟草幼苗后，可以减轻幼苗炭疽病的发病程度；同时烟草幼苗的病原相关蛋白含量有所提高，但只有“巴西”烟草的PR蛋白含量与抗病显著正相关。

Abstract:

Four-leaf seedlings of tobacco were used to study the resistance to anthracnose disease caused by Colletotrichum desructivum O' Gara infection. After or before treatment in seedlings sprayed with 1 mg ml-1 methyl jasmonate (MJ) one time a day for 3 days, fungous spores (108 ml-1) were inoculated to the leaves by spraying 2 times at an interval of 12 hours. The numbers of lesion of leaves were recorded and pathogenesis-related proteins (PRP) were determined after treatment for 7 to 15 days. When tobacco seedlings were inoculated with spores, the resistibility was quite different among three varieties. Brazil variety showed stronger resistance to this disease than K326 and Guanghuang55. Treating with MJ before spore inoculation, the infection symptom in seedlings was reduced, indicating that MJ can induce the resistance of tobacco to fungous infection. The PRP contents of seedlings increased after treating with MJ or inoculating with spores. The correlation coefficient between PRP of Brazil variety and infection degree was -0.9958. It is suggested that PRP is significantly related to fungus resistance in tobacco seedlings of this variety.