金鱼草基因转化和转基因植株再生

GENE TRANSFORMATION AND TRANSGENIC PLANTS REGENERATION OF ANTIRRHINUM MAJUS WITH AGSOBACTERIUM TUMEFACIENS

摘要:

本实验采用根癌农杆菌ＬＢＡ４４０４（ｐ３５ＳＧＵＳＩＮＴ与金鱼草下胚轴切段共培养，将ＧＵＳ基因导入金鱼草细胞，通过不定芽发生途径获得抗Ｇ４１８再生植株．经ＤＮＡ／ＤＮＡ斑点杂交及ＧＵＳ活性原位组织检测初步证实外源基因ＧＵＳ已整合进金鱼草基因组并得到表达．

Abstract:

In this paper, G418-resistant regenerated plants were obtained from hypocotyl segments which had cocultivated with Agrobacterium tumefaciens LBA4404 (p35SGUSINT) via adventitious bud regeneration. DNA/DNA dot hybridization and GUS histochendcal assay revealed that the foreign GUS gene from Agrobacterium tumefaciens LBA4404 had been integrated into genome of Antirrhinum mains and expressed in the cells.