La3+对墨兰根状茎某些细胞器发育的影响

THE EFFECT OF LANTHANUM ON THE DEVELOPMENT OF SEVERAL ORGANELLS IN THE RHIZOME OF CYMBIDIUM SINENSE

摘要:

以１／２ＭＳ＋ＢＡ（１ｍｇＬ－１）＋ＮＡＡ（１ｍｇＬ－１）＋Ｌａ３＋（１０ｍｇＬ－１）培养墨兰根状茎３０ｄ后，取样观察根状茎细胞叶绿体、线粒体和细胞核的发育特点并与对照进行比较，发现：①处理组叶绿体发育较快，有较丰富的片层结构和嗜锇颗粒，而对照组的发育慢，含大型的淀粉粒，无明显的片层结构．②处理组线粒体体积较大，内含物较丰富，而对照组的较小，内含物较少。③处理组细胞核内的染色质较对照组丰富．上述结果表明，ｔａ３＋对墨兰根状茎细胞器的发育有一定的调节作用。

Abstract:

The rhizomes of Cymbidium sinense were incubated in the medium of 1/2MS +BA (1mg L-1) +NAA (1mg L-1) +La3+ (10mg L-1). After incubation for 30d, the rhizomes were used for observating the ultrastructure and development of the chloroplast, ndtochondria and nucleus. The results showed; 1. The development of the chloroplast of the rhizome treated by La3+ was enhanced. There were much more lamellae and osdriophilic granules in chloroplast stroma, and the development of the chloroplast was faster than that of the control. There were little lamellae and larger starch grains in the chloroplast stroma of the control. 2. After treatment with lanthanum, the development of Antochondria was enhanced, and their volume was larger than that of the control. There were more ergastic material in mitochondria stroma of rhizome which was treated with La3+. 3. After treated by La3+, the chromatin in nucleus was increased. The above results showed that there was good effect of La3+ on the development of chloroplast, mitochondria and nucleus in the rhizome of Cymbidium sinense.