吖啶橙失活处理应用于不对称融合初步探讨

INACTIVATION OF MESOPHYLL PROTOPLASTS USING ACRIDIN ORANGE FOR ASYMMETRIC PROTOPLAST FUSION

摘要:

马铃薯栽培种和野生种叶肉原生质体经过吖啶橙（ＡＯ）处理后，暗培养两天，再光照４ｈ而失活．失活程度取决于ＡＯ处理时间、ＡＯ浓度、光照时机等．四倍体失活浓度高于二倍体，处理后马上光照失活效果最强烈，８ｄ后光照其小细胞团同未光照处理相差不大。ＡＯ失活的栽培种ＮＯ７同罗丹明６Ｇ（Ｒ６Ｇ）失活的Ｓｏｌａｎｕｍｂｕｌｂｏｃａｓｔａｎｕｍ融合后可以发生代谢互补恢复分裂，已得到假定的杂种小愈伤组织．

Abstract:

Inactivation of mesophyll protoplasts from potato cultivars and its wild species was achieved by treatment with acridin orange(AO), under dark culture for 2 days and then exposure to light for 4h. The extent of this inactivation depent on the duration of AO treatment, the AO concentrations and the occasion for light treatment. The inactivation concentrations of tetroploid were higher than those of diploid. Immediate exposure to light after AO treatment showed strongest inactivation effect. Culture exposed to light after 8 days showed almost the same division pattern as control AO-treated in the dark. Small calli formed after fusion of protoplasts in rhodamine (P6G) inactivated potato (Solanum tuberosum) and AO-treated Solanum bulbocastanum.