广东两种决明属（Cassia）植物根瘤菌的生物学特性

BIOLOGICAL CHARACTERISTICS OF RHIZOBIUM ISOLATED FROM TWO CASSIA SPECIES FROM GUANGDONG

摘要:

调查了广东地区１０种Ｃａｓｓｉａ植物结瘤状况，仅山扁豆（Ｃ．ｍｉｍｏｓｏｉｄｅｓ）和圆叶决明（Ｃ．ｒｏｔｕｎｄｉｆｏｌｉａ）结瘤，从山扁豆和圆叶决明各分离到１株根瘤菌并鉴定为慢生型．在所试的１０种Ｃａｓｓｉａ植物中，２株Ｃａｓｓｉａ植物根瘤菌除了与山扁豆和圆叶决明结瘤外，均不与其余８种Ｃａｓｓｉａ植物结瘤；同样，３株蝶形花科植物根瘤菌和３株含羞草科植物根瘤菌除了与山扁豆和圆叶决明结瘤外，也均不与其余８种Ｃａｓｓｉａ植物结瘤．此外，２株Ｃａｓｓｉａ植物根瘤菌均能与所试５种蝶形花科和４种含羞草科植物结瘤．２株Ｃａｓｓｉａ植物根瘤菌在碳源利用、耐盐性、耐酸碱度及５种酶的活性等特性方面，与３株蝶形花科植物根瘤菌和３株含羞草科植物根瘤菌极为相似．

Abstract:

Nodulation of ten Cassia species from Guangdong province was surveyed. It wasshown that the nodules could appear only in Cassia mimosoides and C. rotundifolia. Tworhizobial strains were isolated each from C. mimosoides and C. rotundifolia and identifiedas slow-growing Rhizobium. Among ten Cassia species tested, the two rhizobial strains fromCassia failed to nodulate the other eight Cassia species, however, they are capable ofnodulating C. mimosoides and C. rotundifolia. Likewise, three rhizobial strains fromMimosaceae and three from Papilionaceae were unable to nodulate other eight Cassia speciesexcept C. mimosoides and C. rotundifolia. Moreover, the two rhizobial strains from Cassiawere able to nodulate four legumes of Mimosaceae and five legumes of Papilionaceae. Thetwo isolates from Cassia were similar to three isolates from Mimosaceae and three fromPapilonaceae in utilization of carbon sources, salt tolerance, PH tolerance and five enzymeactivities.