水稻雄性不育与花药中类脂褐素的积累

THE MALE-STERILITY OF RICE AND ACCUMULATION OF LIPOFUSCIN-LIKE PIGMENTS IN ANTHERS

摘要:

细胞质雄性不育水稻不育系珍汕９７Ａ和其保持系珍汕９７Ｂ，处于不育期的光（温）敏核不育水稻Ｗ６１５４ｓ和培矮６４ｓ的花药中类脂褐素（ＬＦＬＰ）含量随花粉发育或败育而增高．不育花药中ＬＦＬＰ的形成速率比可育花药快，三核期的珍汕９７Ａ和不育期Ｗ６１５４ｓ的花药，其ＬＦＬＰ比相应具育性花药高２４％．用抗氧化剂ＧＳＨ、ＢＨＴ和Ｎ２处理离体的单核期花药，发现ＧＳＨ可降低珍汕９７Ａ和不育期的Ｗ６１５４ｓ的ＬＦＬＰ含量．结果认为，水稻雄性不育与膜脂过氧化作用的荧光产物类脂褐素的积累有关．

Abstract:

The changes of lipofuscin-like pigment(LFLP)content in anthers of male-sterility rice were studied for examining the possible lipid peroxidative damage in relation to male-sterility.During the pollen development from uninucleate to trinucleate stages, LFLP contents in fertile and sterile anthers all increased gradually to different extents in the tested cytoplasmic male-sterile line rice Zhenshan 97A and its maintainer Zhenshan 97B,the photoperiod(temperature)-sensitive genie male-sterile rice W6154s and Peiai 64s.The LFLP content and accumulative rate in both types of male-sterile rice were higher than those in fertile anthers.In trinucleate stage,the sterile anthers from Zhenshan 97A and W6154s showed 24% higher LFLP content than their fertile anthers.From uninucleate to trinucleate stages of pollen, the average accumulative rates of LFLP in sterile and fertile anthers were 41% and 26% perstage, respectively.When the isolated fresh anthers at uninucleate and trinucleate stages were treated by using N2 and antioxidants glutathione(reduced)and butylated hydroxytoluene,the LFLP content was partially reduced by glutathion treatment at uninucleate stage.The results indicated that the expression of male-sterility of rice was associated with the accumulation of a fluorescent product of lipid peroxidation,lipofuscin-like pigments.