不同活力花生种子子叶内肽酶活性及花生球蛋白的降解

ACTIVITY OF ENDOPEPTIDASE AND DEGRADATION OF ARACHIN IN COTYLEDONS OF GERMINATING PEANUT(ARACHIS HYPOGAEA L.)SEEDS OF VARYING VIGOUR

摘要:

花生种子人工劣变后活力下降，子叶内肽酶活性降低，花生球蛋白降解速率减慢。内肽酶同工酶也发生变化，种子在劣变过程中可能诱导新内肽酶产生。

Abstract:

To determine the activity of endopeptidease and the degradation of arachin in cotyledons of varying vigour peanut seeds during germination, the new harvested peanut seeds(as seeds of high vigour)were stored under relative humility of 95±1% at 28℃ (as seeds of medium vigour)and 40℃(as seeds of low vigour)for 7 days.After the treatment,the vigour index,endopeptidase activity and rate of arachin degradation were reduced.The isoenzyme of endopeptidase also changed.A new isoenzyme exhibited on PAGE in cotyledons of germinating seed of medium vigour on the 3rd to 6th days,and seeds of low vigour on the 6th day.The results showed that the ability of nutrition supply controlled the seed germination, and the endopeptidase played an important role.It was considered that the aging seed syntheses a new endopeptidase isoenzyme in order to accelerate the arachin degradation during seed germination.