杂交稻抗感白叶枯病与活性氧伤害及防御酶系统的关系

STUDY ON THE CORRELATION OF RESISTANCE AND SUSCEPTIBILITY TO BACTERIAL BLIGHT WITH THE ACTIVE OXYGEN AND THE DEFENSIVE ENZYMES IN HYBRID RICE SEEDINGS

摘要:

以对白叶枯病抗感不同的三个杂交稻为材料，在三叶期接种白叶枯病强毒菌株７６—２５，结果表明，杂交稻接种白叶枯病菌７２ｈ后，苯丙氨酸解氨酶（ＰＡＬ）活性和过氧化物酶（ＰＯＸ）活性升高，超氧化物歧化酶（ＳＯＤ）活性下降，膜脂过氧化产物丙二醛（ＭＤＡ）含量增加，且抗病组合的变化幅度大于感病组合，说明杂交稻抗白叶枯病与活性氧伤害及保护酶系的活性相关。

Abstract:

Three hybrid rice combinations, with resistance, susceptibility and hypersensitivity to bacterial blight of rice,namely V6(R),U1/312(S)and S63(HS),were inoculated with Xanthomonas oryzea pv.oryzea 76-25 strain with strong virulence at the three-leaf stage,the uninoculated plants were used as control.The leaves were taken for biochemical analysis after 72 h of inoculation.The results showed that both PAL and POX activities were increased,while the SOD activities were decreased and the MDA content was increased.The changes of all above indexes of resistant combination V6 were more significant than that of susceptiblecombinations U1/312 and S63. However,the decrease of soluble protein content in the leaves of U1/312 and S63 were more significant than that of V6.