大叶相思、马占相思腋芽培养和植株再生

AXILLARY BUD CULTURE AND PLANTLET REGENERATION OF ACACIA AURICULIFORMIS AND A. MANGIUM

摘要:

初选优树上采集的大叶相思、马占相思侧枝芽，经６％次氯酸钠消毒２０ｍｉｎ，０．１％ＨｇＣｌ２消毒５ｍｉｎ后在ＭＳ＋１０μｍｏｌ／ＬＢＡ＋０．５μｍｏｌ／ＬＩＢＡ培养基上进行丛生芽的诱导，结果大芽比小芽更易进行腋芽的诱导。６６．７％的大叶相思大芽，６０．０％的马占相思芽产生了丛生芽。最多的一个大叶相思大芽繁殖了５５个芽，而马占相思最多的是一个外植体繁殖了４８个芽。根的诱导是在１／２ＭＳ无机元素＋ＭＳ有机元素＋４．９２－７．３８μｍｏｌ／ＬＩＢＡ＋２．６９μｍｏｌ／ＬＮＡＡ的培养基上进行的。２９．８％的大叶相思芽苗和２５．０％的马占相思芽苗产生了根，根的诱导还与苗的健壮程度，生理状态有关。继代培养能使植物组织复壮、提高丛生芽的诱导率和促进生根。试管苗经两周锻炼移栽到花盆中，８１．３％的小植株成活并健康生长。

Abstract:

Buds of Acacia auriculiformis and A.mangium from 4-year-old mother trees were first sterilized in 6% chloros for 20 minutes,and then in 0.1% mercuric chloride for 5 minutes.Multiple shoot induction was obtained through culturing on MS basal medium supplemented with 10μmol/L BA and 0.5μmol/L IBA.Large explants were easier to induce multiple shoots than small ones.66.7% of large explants of A. auriculiformis and 60.0% of A.mangium explants produced multiple shoots,the largest shoot number per explant reached 55 and 48 respectively.Root induction was done on half concentration of MS inorganic compounds supplemented with formal MS organic compounds, 4.92-7.38μmol/L IBA and 2.69μmol/L NAA medium.Rooting percentages of A.auriculiformis and A. mangium shoots were 29.8% and 25.0%,respectively.The frequency of rooting also related with shoot's health and its physiological state.Subculture can rejunvenate plant tissue,increase the number of shoot per explant and promote rooting.Vigorous plantlet can be obtained when the rooted-shoots were transplanted to soil after two-week training.