水稻广亲和基因（WCG）近等基因系的随机引物PCR分析

RAMDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF NEAR-ISOGENIC LINES OF WIDE COMPATIBILITY GENE IN RICE

摘要:

应用随机引物ＰＣＲ（ＲａｎｄｏｍＰｒｉｍｅｒＰＣＲ）技术分别在水稻广亲和基因（ＷＣＧ）的近等基因系和色素原基因（Ｃ）的分离群体库中寻找与ＷＣＧ和Ｃ基因连锁的分子标记。对于ＷＣＧ近等基因系，在２２６个随机引物中初筛到２２个显示多态性片段的引物。根据理论值计算，在２２个多态性片段中预期有２０个与ＷＣＧ连锁。在这些连锁标记中距ＷＣＧ最近的可达０．５ｃＭ。同样在分离群体库的筛选中有１０个扩增产物与Ｃ基因连锁。

Abstract:

There are growing evidences that the fertility of hybrid between indica and japonica varieties of rice (Oryza sativa L. )is controlled mainly by a wide compatibility gene (WCG), which is closely linked to the chromogen gene (C gene ). Screening new molecular markers nearby WCG and C gene by RAPD will be an effective approach to enrich the present molecular map. Some results obtained from RAPD analysis and bulked segregants of C gene for Near Isogenic Lines (NILs) of WCG are shown. By method of polymerase chain reaction (PCR), 22 polymorphic primers in 226 random primers are found for NILs of WCG, among which 20 polymorphic DNA fragments should be linked with WCG under theoretical calculation, and the expected minimum map distance is 0. 5 cent morgan. By using bulked segregants of C gene, 10 polymorphic DNA fragments in 200 random primers are found out.