光周期诱导光敏感核不育水稻花药蛋白变化的研究

STUDY ON CHANGES OF PROTEIN IN ANTHERS OF PHOTOPERIOD-SENSITIVE GENIC MALE-STERILE RICE DURING PHOTOPERIODIC INDUCTION

摘要:

采用双向电脉技术对不同光周期条件下，光敏感核不育水稻（农垦５８ｓ）的可育与不育花药蛋白的变化进行分析，发现花粉发育的不同阶段中，不育花药具有四个特异蛋白ｐＩ６．２／ｂＭＷ７０ＫＤ，ｐＩ６．２／ＭＷ６８ＫＤ，ｐＩ６．２／ＭＷ３８ＫＤ和ｐＩ７．４／ＭＷ３７ＫＤ．对游离组蛋白的分析表明．长日照诱导的不育花药中游离组蛋白的相对百分率均明显低于短日照下的可育花药．据此推测长日照诱导不百花药蛋白质组成和代谢变化．不育花药中游离组蛋白含量低，可能受ＤＮＡ合成数量少的影响．

Abstract:

The changes of anther proteins in a typical Hubei Photoperiod-sensitive genie male -sterile rice (HPGMR) Nongken 58s under the induction of long day (14/10h, light/dark) and short day (9/15h, lisht/dark) were analysed by two-dimensional polyacrylamide gel electrophoresis. Proteins from anthers were resolved into 180 components.The protein electrophoretogram showed that there were evident difference between sterile anthers and fertile anthers at diffent stages of pollen development. Four of the protein spots, namely P1 (70KD, pI6. 2), P2 (68KD,pI6. 2), P3 (37KD, pI7. 4) and P7 (38KD, pI6. 2) were oborved in sterile anthers from early uninucleate stage to trinucleate stage of pollen. These proteins were considered as the specific proteins related to sterility formation under iong day induction.Free histone was also analysed by SDS-PAGE in anthers. It showed that the histone patterns were chansed with the development of pollen, and the relative contentS of free histone in sterile anther were lower than that in fertile anther by 19. 6% and 7. 4% at the uni- and dinucleate stages of pollen. The decrease of Part of flee histone content may be caused by the effects of decreasing amount of synthetic DNA in the sterile anthers.