花生胚离体发育及渗调物质的影响

IN VITRO DEVELOPMENT OF PEANUT EMBRYO: EFFECT OF OSMOTIC REGULATOR

摘要:

在无外源激素培养基上花生胚能继续发育．渗调物质如甘露醇可抑制胚早萌，维持胚性发育，促进贮藏蛋白质合成和累积．渗调物质对胚离体发育的调控与其提高胚内源ＡＢＡ含量有关．

Abstract:

In this paper, endogenous ABA content, growth, Precocious germination, storage protein accumulation of peanut (Arachis hypogaea L. cv. Yueyou-116) embryo in vitro wereexamined. Four treatments in the experiment were given as follows: (A)embryo with seed coat +8% mannitol; (B) embryo with seed coat+no mannitol; (C) embryo withoutseed coat + 8% mannitol; (D) embryo without seed coat + no mannitol. The resultsshowed that the embryo of peanut could normally develop without exogenous hormones.The osmotic regulator such as mannitol inhibited precocious germination, maintained embryonic development and promoted the synthesis and accumulation of storage protein. Endogenous ABA content rose in the treatment with mannitol in vitro. The regulatory effectof mannitol on the development of embryo in vitro was probably related to the incerase ofendogenous ABA content in embryo tissues.