黄花蒿组培快繁与种质离体保存的研究

Rapid Propagation and Germplasm Conservation in vitro of Artemisia annua L.

摘要:

以带侧芽的黄花蒿(Artemisia annua L.)茎段为外植体，以MS为基本培养基，进行组织培养和种质保存研究。结果表明，培养基MS+6-BA 1.0 mg L-1+IBA 0.1 mg L-1、MS+6-BA 0.5 mg L-1+IBA 0.1 mg L-1和MS+NAA 0.1 mg L-1+IBA 0.5 mg L-1可分别用于黄花蒿的芽诱导、增殖和生根培养，培养20 d的增殖倍数为5.5倍，生根率98.3%。 培养基MS+CCC 1.0 mg L-1、MS+CCC 2.0 mg L-1、MS+PP333 4.0 mg L-1可用作离体保存, 连续保存200 d的存活率分别达72.3%、77.0%、69.2%。活力检测表明，黄花蒿种质经保存后增殖、生根能力没有下降。因此，可通过诱导腋芽增殖建立黄花蒿快繁体系，及在培养基中添加CCC或PP333能使材料长期保存。

Abstract:

The rapid propagation and germplasm conservation in vitro of Artemisia annua L. were studied using stem with axillary buds as explants cultured on Murashoga and Skoog (MS) basal medium. The buds could be induced successfully on MS medium supplemented with of 1.0 mg L-1 6-BA and 0.1 mg L-1 IBA. The suitable medium for bud multiplication was MS medium supplemented with 0.5 mg L-1 6-BA and 0.1 mg L-1 IBA. The buds coulde be proliferated by 5.5 times cultured for 20 days. The rooting medium was MS medium with 0.1 mg L-1 NAA and 0.5 mg L-1 IBA, accounting for 98.3% of rooting. The MS medium supplemented with CCC or PP333 were found to be suitable for germplasm conservation in vitro. After preserved on MS+ CCC 1.0 mg L-1, MS+CCC 2.0 mg L-1 and MS+ PP333 4.0 mg L-1 for 200 days, the survival rate of germplasm get to 72.3%, 77.0% and 69.2%, respectively, and the ability of propagating and rooting of the plantlets was not decreased. All these results indicated that the rapid propagation system of A. annua had been established by inducted axillary buds, and the germplasm could be conserved for a long time on MS medium supplement with CCC or PP333.