水稻(Oryza sativa L.)重复DNA顺序的克隆及序列分析——重复顺序存在意义的探讨

CLONING AND SEQUENCE ANALYSIS OF REPETITIVE DNA FRAGMENT FROM RICE ( ORYZA SATIVA L. ) A Proposed Role of Repeated DNA in Genome

摘要:

以大肠杆菌启动子选择质粒pKK175-6、pKK232-8为载体,将通过复性动力学方法获得的水稻重复DNA顺序片段进行克隆。阳性克隆菌株表现出对四环素或氯霉素不同程度的抗性。DNA序列分析表明,克隆到的某些水稻重复DNA顺序具有丰富的基因表达调节元件的同源序列。

Abstract:

By application of appropriate vectors together with DNA renaturation kinetic method, repetitive DNA sequences of different Cot value were cloned from rice ( Oryza sativa L. ) genome. Two promoter selection plasmids, pKK175-6 and pKK232-8, were used as cloning vectors, which contained promoters Tet or Cat gene, respectively. The recombinant clones acquired resistance to Tc or Cm, and their resistant levels varied from 20μg/ml to 80μg/ml Tc or 30μg/ml to 260μg/ml Cm. The sequence analysis showed that there we...