低压脉冲电泳介导的外源基因转化部分酶解的水稻小细胞团

ELECTRQPHORETIC DRIVE: HIGH EFFICIENCY GENE TRANSFER USING A NOVEL APPROACH FOR THE DELIVERY OF FOREIGN GENES INTO RICE CELLS

摘要:

开发了一类新型的低压脉冲电泳法介导外源基因进入水稻细胞的转化系统。本系统以水稻部分酶解小细胞团为受体,采用低压脉冲电泳推动质核DNA进入水稻细胞。以报告基因GUS酶活性为指标,借以测定转化了的水稻细胞。最佳的组合处理可以获得8.2％的转化频率。文中对低压脉冲电泳转移外源基因的条件亦作了讨论。

关键词:水稻;小细胞团;电泳;基因转化

Abstract:

A new method has been developed to introduced foreign DNA into rice cells. Gene delivery occurs when a low-voltage electrophoresis at long pulse applied to a mixture containing partially digested SCG (small cell groups) and plasmid DNA. Gene-transfer efficiency is evaluated by a detection of GUS activity resulting from the chimeric plasmid DNA. The optimal combination of voltage and pulse produces a frequency of up to 8. 2% for transformation of SCG. The results demonstrates that electrophoretic drive is applicable to introduce foreign genes into plant cells.

Key words:Rice;Small cell groups (SCG) , Electrophoresis;Gene transfer;Stable expression