几种海藻和赤潮藻的DCMU增益荧光比率及其与增殖速率的关系

DCMU-ENHANCED FLUORESCENCE RATIO AND ITS RELATION TO GROWTH RATE IN VARIOUS SEAWEEDS AND RED TIDE SPECIES

摘要:

本文研究了红藻梅膜(Halymenia sinensis),绿藻孔石莼(Ulva pertusa),褐藻裙带(Underia pinnatifida)及蓝藻钝顶螺旋藻(Spir~dulina platensis)的DCMU增益荧光比率(F_D/F_N)。其中海膜的比率最高(3.9),石莼及螺旋藻次之(2.5和2.8),裙带(采自夏季)的比率最低(1.7)。脱水及热处理下和藻体老化时,F)D/F_N值显著下降。在实验室条件下分批培养的两种赤潮藻海洋原甲藻(Prorocentrum micans)和微小角毛藻(Chaetocersa minutissmus),其增殖过程中最大增益荧光比率较最大增殖速率出现早,这一结果为赤潮的预报提供了依据。

Abstract:

DCMU-enhanced chlorophyll fluorescence ratio of marine algae [Halymenia sinensis (red alga), Ulva pertusa (green'alga), Underia pinnatifita (brown alga) and Spirulina platensis (blue-green alga)] was studied. It was found that the red alga had the highest ratio (3. 9) among these species. The green one and blue-green alga had the value of 2. 5 and 2. 8 respectively. The ratio for U. pinnatifida (collected in summer season) was as low as 1. 7. FD/FN values declined obviously under the effects of stresses (dehydration or heat). If also reduced when the algae senesced. So this ratio can be used as a sensitive index of photosyhthetic capacities of marine algae. In addition, the FD/FN values of two phytoplanktons (red tide species) Prarocentrum micans and Chaetoceras minutissmus both in batch culture, and their relation to growth rate were investigated. The resnlts indicated that both FD/FN value and U changed with the time of culture, but the important fact was that the maximum value of fluorescence ratio was reached always prior to the appearing of the maximum of growth rate. This results presented a good prospect for using fluorescence in vivo in the research of red tide occurence.