Abstract:The Nabanhe National Nature Reserve is situated at Xishuangbanna, the most southern part of Yunnan Province, China. Based on the field survey and identification of about 1 300 collected specimens, 59 families, 157 genera, 385 species, 3 subspecies and 3 varieties of bryophytes were first reported from the Nabanhe National Nature Reserve. Of these, 3 species are newly recorded to mainland of China, 2 genera and 12 species or variety to Yunnan Province, and 4 families, 12 genera and 140 species to Xishuangbanna Region. In addition, the composition of the dominant genera and species and types of habitats of the bryophytes in the Reserve are also analyzed and discussed. The results provided useful data and information for flora studying of bryophytes in tropical region of China.