百合花瓣衰老过程中的蛋白质变化研究

Changes in Protein during Senescence of Lily Cut Flowers

摘要:

以5个百合品种为材料，对百合切花花瓣衰老过程中可溶性蛋白的变化进行了研究。结果表明，5个品种的百合切花都具有相同的蛋白质条带，如49.9 kDa、45 kDa、32.8 kDa、22.1 kDa；但品种间也有明显差异，如38.4 kDa是‘黄色风暴’特有的；40 kDa为‘铁炮百合’特有；‘金百合’没有26.5 kDa的条带，但是相同的杂交品系之间蛋白条带的相似性要明显高于不同杂交品系；在百合花花瓣衰老过程中有的蛋白质的含量不断下降，而有的保持不变；在花瓣衰败期有新的蛋白条带出现，如‘西伯利亚’、‘黄色风暴’和‘索邦’均出现58.3 kDa条带、‘金百合’出现67 kDa的条带，这些条带可能与花瓣衰老相关，是衰老特异蛋白。

Abstract:

The changes of proteins in cut flowers of five cultivars of lily (Lilium spp.) during senescence were studied using the improved polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that there were some similar protein bands, i.e. 49.9 kDa、45 kDa、32.8 kDa、22.1 kDa, in five lily cultivars, but there also were clear difference among the varieties, for example, the band of 38.4 kDa was characterized for ‘Aziatische Gr. Yelloween’, ‘Longiflorum’ with 40 kDa, but no 26.5 kDa. However, the similarity of protein band between the same hybrid strains was obviously higher than that between different ones. During the process of senescence, the contents of some protein decreased constantly, but some keep stablity. There were new protein bands occurred during petal degrade stage, such as 58.3 kDa for ‘Oriental Gr. Siberia’, ‘Oriental Gr. Sorbonne’, and ‘Aziatische Gr. Yelloween’, 67 kDa for ‘Aziatische Gr. Trompeten’. It suggested that these specific proteins were related to petal aging possibly.