中国菟丝子离体再生体系的建立

Plant Regeneration in vitro of Cuscuta chinensis Lam.

摘要:

选取萌发3~5 d、长度3~5 cm的中国菟丝子(Cuscuta chinensis Lam.)幼苗，将其分为上、中、下3部分并作为外植体进行离体培养与植株再生研究。结果表明，其上、中部片段更适宜愈伤组织诱导；诱导培养基以添加1 mg L-1 NAA和1 mg L-1 BA的MMS培养基效果最好，此培养基也可用于愈伤组织的继代培养；愈伤组织在上述培养基中已生长一年之久。分化培养基为添加1 mg L-1 BA的MMS培养基，平均每块愈伤组织可以产生2.8株植株。

Abstract:

An efficient method of callus induction and plant regeneration of Cuscuta chinensis was established. The seedlings germinated for 3~5 d and about 3~5 cm length were selected as explants, and then divided into three parts, upper, middle and lower. Callus were inducted from upper or middle parts of seedlings on a modified Murashige and Skoog (MMS) medium supplemented with 1 mg L-1 NAA and 1 mg L-1 BA. The calli have been subcultured on such medium for over a year. Shoot regeneration from callus was achieved on MMS medium containing 1 mg L-1 BA and could obtain 2.8 shoots per callus.