组培广藿香形态特征及挥发油成分分析

Morphological Characters and the Composition of Essential Oil from Regeneration Plant of Pogostemon cablin

摘要:

对原产于广州石牌和海南的两个广藿香(Pogostemon cablin)栽培品种进行组织培养并获得了再生植株。用GC法对广藿香再生植株的挥发油成分进行分析研究。结果表明，广藿香两个品种的再生植株生长2~3个月后形态上有明显的差异。生长3个月的再生植株中，原产于广州石牌的广藿香挥发油含量为1.4%，低于原产于海南的广藿香（2.9％）；而挥发油的成分中，广州石牌广藿香的广藿香酮含量为375.76 mg ml-1，显著高于海南广藿香（7.82 mg ml-1）。这说明组织培养获得的再生植株保持了其原植物在形态、挥发油含量和成分上的差异性，为广藿香的品种分类提供了实验依据。

Abstract:

Calli from leaf explants of two cultivars of Pogostemon cablin (Blance) Benth., which derived from Shipai in Guangzhou and Hainan, respectively, were obtained on Murashige Skoog (MS) medium supplemented with 2,4-D (0.05 mg L-1) and KT(0.5 mg L-1), and plantlets regenerated through callus were obtained on MS medium supplemented with 6-BA (1 mg L-1). Essential oils were extracted from the regenerated plant and the constituents were analyzed using GC method. The results showed that there were obviously morphological differences in regenerated plants of the two cultivars after cultured for 2~3 months. Essential oil content in cultivar from Shipai was 1.4％，lower than that from Hainan, which was 2.9%, but the content of pogostone in cultivar of Shipai, being 375.76 mg ml-1, was significantly higher than that from Hainan, which was 7.82 mg ml-1. These differences exsited in the initial plants. This results provided more scientific basis for the classification of Pogostemon cablin.