木瓜蛋白酶的固定化及其性质研究

Studies on Immobilization of Papain and the Properties of Immobilized Enzyme

摘要:

在海藻酸钠-壳聚糖固定化木瓜蛋白酶(immobilized papain on sodium alginate-chitosan,IPSAC)的实验中，当给酶量为1 mg g-1载体时，酶活性为39.2 U，酶活力回收为21.1％。在尼龙布固定化木瓜蛋白酶(immobilized papain on nylon，IPN)的实验中，当每块尼龙布(3 cm×3 cm)给酶量为1 mg时，酶活性为35.6 U，酶活力回收为19.2％。木瓜蛋白酶(papain, PA)、IPSAC、IPN的最适pH分别为7.2、7.2和6.8。PA及IPSAC在70℃以下活性稳定；IPN在50℃以下活性稳定。IPSAC与IPN半衰期分别为59 d和66 d。

Abstract:

Papain was immobilized on sodium alginate-chitosan and nylon, respectively. The immobilization conditions and characterization of the immobilized enzyme were studide. When 1 mg g-1 carrier papain (PA) was loaded on sodium alginate-chitosan (IPSAC), the IPSAC activity was 39.2 U, and the recovery of activity was 21.1%. As PA was loaded 1 mg on one block nylon (3 cm×3 cm), the IPN activity was 35.6 U, and the recovery of activity was 19.2%. The optimum pH of PA, IPSAC and IPN were 7.2, 7.2 and 6.8, respectively. PA and IPSAC were stable when the temperature was lower than 70℃, while IPN was stable below 50℃. The half-life of IPSAC and IPN were 59 d and 66 d, respectively.